Effect of twine thickness on selectivity of gillnets for bogue, Boops boops,in Turkish waters

To investigate the effect of twine thickness on the selectivity of multifilament gillnet targeting bogue,Boops boops L., four different stations were sampled between March and November 2008 in the northern Aegean Sea. Gillnets with 22, 23, and 25 mm nominal mesh size (bar length) each having two different twine thicknesses (approximately 0.45 mm and 0.54 mm ∅) were applied for this purpose. The deviances from the SELECT method revealed that lognormal models provided the best fits for both of the twine thicknesses. Results from the two-way ANOVA analyses revealed that the mean total lengths increased with the mesh size (F = 87.36; df = 2; P < 0.0001) and decreased with the twine thickness (F = 46.12; df = 1; P < 0.0001). The 22 mm mesh size net (0.45 mm ∅) captured significantly larger fish than the 23 mm mesh size net (0.54 mm ∅) probably due to the higher elasticity and flexibility of the thinner twine. Thus, fisheries managers should take into consideration the twine thickness while advising mesh size regulations in gillnet fisheries

Modelling and Simulation of a Manipulator with Stable Viscoelastic Grasping Incorporating Friction

Design, dynamics and control of a humanoid robotic hand based on anthropological dimensions, with joint friction, is modelled, simulated and analysed in this paper by using computer aided design and multibody dynamic simulation. Combined joint friction model is incorporated in the joints. Experimental values of coefficient of friction of grease lubricated sliding contacts representative of manipulator joints are presented. Human fingers deform to the shape of the grasped object (enveloping grasp) at the area of interaction. A mass-spring-damper model of the grasp is developed. The interaction of the viscoelastic gripper of the arm with objects is analysed by using Bond Graph modelling method. Simulations were conducted for several material parameters. These results of the simulation are then used to develop a prototype of the proposed gripper. Bond graph model is experimentally validated by using the prototype. The gripper is used to successfully transport soft and fragile objects. This paper provides information on optimisation of friction and its inclusion in both dynamic modelling and simulation to enhance mechanical efficiency

ANTICARCINOGENIC ACTIVITY OF RICE BRAN PHYTIC ACID AGAINST HUMAN BREAST CANCER CELL LINE (MCF-7)

Phytic acid (PA) has been reported for anti-inflammatory, antioxidant and anticancer activity. However, molecular mechanism of anticancer activity is not clear. This study investigated the anticancer activity of rice bran PA against breast cancer (MCF-7). Cytotoxicty of PA (0 to 7 mM) against MCF-7 cells was examined by MTT and LDH assays after 24 and 48 h treatment. Apoptotic activity was evaluated by expressional analysis of apoptosis-regulatory genes [i.e., p53, Bcl-2, Bax, caspase-3 and -9] by reverse transcriptase-PCR and DNA fragmentation assay. PA inhibited the growth of MCF-7 cells in a concentration dependent manner (p≤0.04). After 48 h treatment, cells viability was recorded 80.9, 71.1, 59.8, 36.6, 26.7 and 15.9% in MTT assay and 85.3, 72.6%, 62.3%, 42.1, 31.7 and 21.7% in LDH assay at concentration of 1.4, 2.2, 3.0, 3.8, 4.6, and 5.4 mM respectively. Hence, treatment of PA for 24 h, recorded viability of cells 84.6, 73.8, 61.0, 47.0, 28.8 and 17.3% in MTT assay and 87.8, 77.5%, 62.9%, 49.8, 35.7 and 23.3% in LDH assay at concentration of 2, 3, 4, 5, 6, and 7 mM, respectively. PA treated MCF-7 cells showed up-regulation of p53, Bax, caspase-3 and -9, and down-regulation of Bcl-2 gene (p ≤0.03). At IC50 (3.4 mM) of PA, the p53, Bax, caspase 3 and -9 genes were up-regulated by 6.34, 4.90, 23.45 and 15.03 folds respectively. Also, the fragmented genomic DNA in PA treated cells showed the signs of apoptosis. Our study endorsed the biological activity of PA and demonstrated the PA induced growth inhibition and apoptosis in MCF-7 cells by modulating the expression of apoptosis-regulatory genes. Keyword: Phytic acid, antioxidant, cytotoxicity, apoptosis, caspases, p53, Bax, Bcl-2, DNA fragmentatio

Target Product Profiles for medical tests: a systematic review of current methods

Background: A Target Product Profile (TPP) outlines the necessary characteristics of an innovative product to address an unmet clinical need. TPPs could be used to better guide manufacturers in the development of ‘fit for purpose’ tests, thus increasing the likelihood that novel tests will progress from bench to bedside. However, there is currently no guidance on how to produce a TPP specifically for medical tests. Methods: A systematic review was conducted to summarise the methods currently used to develop TPPs for medical tests, the sources used to inform these recommendations and the test characteristics for which targets are made. Database and website searches were conducted in November 2018. TPPs written in English for any medical test were included. Based on an existing framework, test characteristics were clustered into commonly recognised themes. Results: Forty-four TPPs were identified, all of which focused on diagnostic tests for infectious diseases. Three core decision-making phases for developing TPPs were identified: scoping, drafting and consensus-building. Consultations with experts and the literature mostly informed the scoping and drafting of TPPs. All TPPs provided information on unmet clinical need and desirable analytical performance, and the majority specified clinical validity characteristics. Few TPPs described specifications for clinical utility, and none included cost-effectiveness. Conclusions: We have identified a commonly used framework that could be beneficial for anyone interested in drafting a TPP for a medical test. Currently, key outcomes such as utility and cost-effectiveness are largely overlooked within TPPs though and we foresee this as an area for further improvement

Time to focus on outcome assessment tools for childhood vasculitis

Childhood systemic vasculitides are a group of rare diseases with multi-organ involvement and potentially devastating consequences. After establishment of new classification criteria (Ankara consensus conference in 2008), it is now time to establish measures for proper definition of activity and damage in childhood primary vasculitis. By comparison to adult vasculitis, there is no consensus for indices of activity and damage assessment in childhood vasculitis. Assessment of disease activity is likely to become a major area of interest in pediatric rheumatology in the near future. After defining the classification criteria for primary systemic childhood vasculitis, the next step was to perform a validation study using the original Birmingham vasculitis activity score as well as the disease extent index to measure disease activity in childhood vasculitis. Presently, there are efforts in place to develop a pediatric vasculitis activity score. This paper reviews the current understanding about the assessment tools (i.e., clinical features, laboratory tests, radiologic assessments, etc.) widely used for evaluation of the disease activity and damage status of the children with vasculitis. © 2011 Demirkaya et al; licensee BioMed Central Ltd

A case of chronic recurrent multifocal osteomyelýtýs successfully treated with etanercept

PubMe

Using MazeSuite and Functional Near Infrared Spectroscopy to Study Learning in Spatial Navigation

MazeSuite is a complete toolset to prepare, present and analyze navigational and spatial experiments1. MazeSuite can be used to design and edit adapted virtual 3D environments, track a participants' behavioral performance within the virtual environment and synchronize with external devices for physiological and neuroimaging measures, including electroencephalogram and eye tracking

Effects of Vitamin D levels on asthma control and severity in pre-school children

OBJECTIVE: Prevalence of asthma and Vitamin D deficiency has been increasing and leading to significant morbidities. This study aimed to compare the Vitamin D levels in the pre-school children with asthma and in healthy controls and to assess the relationship between Vitamin D levels and asthma clinical parameters and control. PATIENTS AND METHODS: Vi tamin D [25(OH)D3] levels were measured in 102 preschool children, aged 1-4 years with asthma and 102 healthy controls in winter. The patients with asthma were grouped according to serum Vitamin D levels as sufficient, insufficient and deficient. Asthma control was classified according to the Global Initiative for Asthma (GINA) guidelines and the Test for Respiratory and Asthma Control in Kids (TRACK) in 1-4 years-old children. RESULTS: Serum Vitamin D levels were 22.64 (9.96) ng/ml in the asthma group and 32.11 (14.74) ng/ml in the control group (p = 0.001). Total number of exacerbations during the previous year were significantly lower in the Vitamin D sufficient group, compared to the deficient and insufficient groups (p = 0.03). Frequency of patients with controlled asthma was higher in the sufficient group compared to the deficient and insufficient groups (p = 0.001 and p = 0.001, respectively). There was a positive correlation between serum Vitamin D levels and asthma control. CONCLUSIONS: The frequency of Vitamin D deficiency and insufficiency was higher in children with asthma, compared to the controls. Therefore, we suggest that lower levels of Vitamin D are associated with poor asthma control and increased asthma severity

Breeding performance of sustainable fish Ctenopharyngodon idella through single intramuscular injection of Ovaprim-C at Bahawalpur, Pakistan

Effect of intramuscular injection of Ovaprim–C on the number of eggs/kg,  fertilization rate and hatching percentage were studied at a private Fish Hatchery at Bahawalpur, Pakistan, during April to June 2008, on Ctenopharyngodon idella (Grass carp). Studied fish specimens were spawned successfully following a single dose of injection of Ovaprim-C (LH-RH analogue) with 0.6 ml kg-1 for female and 0.2 ml kg-1 for male brooders. Ova and milt were stripped simultaneously and mixture was stirred for 15 to 30 s during which fertilization occurred. Hatching occurred within 18 to 30 h after fertilization. The experiment was conducted in circular spawning tank with 2 m diameter. It was observed that body weight has positive influence on absolute fecundity (r = 0.967), while relative fecundity remained constant with increasing body weight. If it is impossible to determine the absolute and relative fecundity then these parameters can be determined from the body weight.Key words: Induced spawning, Ovaprim-C, fecundity, Ctenopharyngodon idella

Association of vitamin D deficiency and disease activity in systemic lupus erythematosus patients: Two-year follow-up study

Objectives: This study aims to determine the prevalence of vitamin D deficiency in Pakistani systemic lupus erythematosus (SLE) patients and the effect of vitamin D deficiency on the severity and outcomes of SLE.Patients and methods: This retrospective study evaluated SLE patients presenting to our hospital between January 2009 and December 2018. A total of 98 patients (13 males, 85 females; mean age 39.8±14.9 years; range, 16 to 73 years) with vitamin D levels available at the time of diagnosis were included in the study. Disease activity was measured using SLE disease activity score at the time of diagnosis and at the two-year mark.Results: Sixty-five patients were deficient in Vitamin D and out of those 46 were severely deficient. The severe disease group had more patients with vitamin D deficiency at both visits (43/78 and 33/46) while patients in remission all had normal vitamin D (12/12 and 14/14) (p≤0.001).Conclusion: Vitamin D deficiency is common in SLE patients and also significantly associated with increased disease activity at the time of diagnosis and at the two-year mark. We hope this study becomes a platform for the global medical community to come together and implement early screening and monitoring of vitamin D levels and to determine the optimal level of supplementation for prevention of poor outcomes in SLE